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last update time 2017/09/14
:::* Home > Professor > Wen-Lung Chen

** Professor

Wenlung Cheng  教授近照
Title: Professor
Education: Ph.D. Georgia Institute of Technology (1992)
Research Area: Analytical Chemistry, Biophysical Chemistry
Tel: 886-5-271-7965


Research Interests:

We are interested in the development of Raman Techniques (Dispersive Raman, FT-Raman, and Surface Enhanced Raman Scattering) to explore bioactive molecules of natural products and to characterize molecular structure of biomacromolecules. Raman spectroscopy is well known for its capability of providing rich molecular information. However, the application of Raman technique in studying natural pigments is usually hampered by the strong fluorescence signals arising from the intrinsic chromophores/fluorphors of natural pigments in plant. To overcome this problem, the development of Fourier transform Raman spectroscopy (FT-Raman) excited at near-infrared wavelength has demonstrated tremendous breakthrough. Although FT-Raman has made much progress in relaxing fluorescence interference and also has exhibited much spectroscopic superiority such as simple, direct, and free from photo bleaching due to the advantages of near infrared excitation, and the high throughput and multiplex characteristics of Michelson interferometer, it remains suffering from the shortcoming of low sensitivity, particularly for the solutions at low concentration. Therefore, to develop a high sensitive Raman technique to characterize nature pigments remains an important issue. With the aid of nano technique, we are developing surface-enhanced Raman scattering (SERS) to be an excellent ultra sensitive tool in probing trace molecules of natural pigments such as catechins and anthocyanins, of which Raman signals are usually overwhelmed by the strong fluorescence arising from their intrinsic chromophores/fluorphors. Research projects for this part include: (1) Separation, purification, and identification of bioactive compounds from Yam (2) Raman spectroscopic study of natural pigments such as catechin and anthocyanin (3) Developing ultrasensitive Raman detection system.

The second part of our research goal is to characterize protein structure by Raman spectroscopy. We have worked on eye lenses and lens proteins for years. Understanding the factors of various protein perturbations and to prevent lens from oxidative insults will lessen cataract formation that is a result of protein aggregates and remains an unsolved problem in human being. There remains no efficient method to diagnose cataract formation at its early stage, as a noninvasive tool Raman spectroscopy sheds some light on probing cataract at its early stage. The ongoing research includes purification of lens crystallins from eye lenses and characterization of their structures by Raman spectroscopy.




1.       W. Chen, I. M. Weung, and C. Y. Tseng (2003) Antioxidative and antimutagenic activities of healthy herbal drinks from Chinese medicinal herbs, Amer. J. Chin. Med. 31, 4, 1-10. SCI


2. C. L. Shiu, W. Chen, I. M. Weng, and C. Y. Tseng (2003) Chemical composition, physical properties and antioxidant activities of yam flours as affected by different drying methods., Food Chem., 83. 85-92. SCI


3. Y. M. Weng, R. H. Weng, C. Y. Tzeng, and W. Chen* (2003) Structural Analysis of Triacylglycerols and Edible Oils by Near-Infrared Fourier Transform Raman Spectroscopy. Applied Spectroscopy, 57, 4, 413-418. SCI


4. C. H. Huang, R.J. Chang, S. L. Huang, and W. Chen (2003) Dietary Vitamin supplementation affects tissue lipid peroxidation of hybrid tilapia, Oreochormis niloticus x O. aureus’. Comp. Biochem. and physiol, 134, 265-270.


5. S. H. Shih, Y. M. Weng, S. L. Chen, S. L. Huang, C. H. Huang, and W. Chen* (2003) FT-Raman Spectroscopic Investigation of Lens Proteins of Tilapia Treated with Dietary Vitamin E. Arch. Biochem. & Biophys., 420, 79-86. SCI


6. C. L. Hsu, S. L. Hurang, W. Chen, and Y. M. Weng, and C. Y. Tseng (2004) Qualities and antioxidant properties of bread as affected by the incorporation of Yam flour in the formulation. Internal. J. Food Sci. & Tech., 39,231-238. SCI


7. L. Y. Kuo, G. Y. Huang, S. L. Yang, Y. W. Hua, W. Chen, and L. L. Lin (2004) Inactivation of Bacillus stearothermophilus leucine aminopectidase II by hydrogen peroxide and site-directed mutagenesis of methionine residues on the enzyme. Protein J., 23, 295-302. SCI


8. M. C. Chi, W. M. Chou, C. H. Wang, W. Chen, W. H. Hsu, and L. L. Lin (2004) Generating oxidation-resistant variants of Bacillus kaustophilus leucine aminopeptidase by substitution of the critical methionine residues with leucine. J.Gen. & Appl..Microbiol. 1-8. SCI


9. Y. H. Liao, C. H. Wong, C. Y. Tseng, H. L. Chen, R. L Lin, and W. Chen* (2004) Compositional and Comformational Analysis of Yam Proteins by Near Infrared Fourier Transform Raman Spectroscopy. J. Agric. & Food Chem. 52, 8190-8196. SCI


10. B. L Hsu, I. M. Weng, Y. H. Liao, and W. Chen* (2005) Structural investigation of edible zein films/coatings and directly determining their thickness by FT-Raman spectroscopy. J. Agric. & Food Chem. 53, 5089-5095 SCI


11. Y. H. Liao, C. Y. Tseng, and W. Chen* (2006) Structural characterization of dioscorin, the major tuber protein of yams, by near infrared Raman spectroscopy. J. Physics: conf. ser., 28, 119-122.


12. R. H. Weng, I. M. Weng,. C H. Wong, K. L. Ku, and W. Chen* (2006) Authentication of Camellia oleifera Abel Oil by Near Infrared Fourier Transform Raman Spectroscopy. J. Chin. Chem. Soc. 53, 1-7. SCI


13. P. Y. Chou, C. H. Hong, W. Chen, Y. J. Li, Y. S. Chen, R. Y.-Y. Chiou (2006) A glass distilling collector applied for HCN recovery from submerged-cultivated broth and fruiting body of Pleurotus eryngii for identification and quantification. J. Agri. & Food Chem. 54, 1551-1556. SCI


14. M. Liang, V. Chen, H. L. Chen, and W. Chen*(2006) A simple and direct isolation of whey components from raw milk by gel filtration chromatography and structural characterization by Fourier transform Raman spectroscopy. Talanta 69, 1269-1277. SCI


15. H. L. Yang, R. S. Chen, W. Chen, L. L. Lin (2006) Identification of glutamate residues important for catalytic activity of Bacillus stearothermophilus leucine aminopeptidase II. Antonie van Leeuwenhoek Inter. J. Gen. & Mole. Microbiol. 90,195-199 SCI


16. C. T. Liu, P. T. Chuang, C. Y. Wu, Y. M. Weng, W. Chen, and C. Y. Tseng (2006) Antioxidative and in vitro hepatoprotective activity of bupleurum kaoi leaf infusion. Phytother. Res. (in press) SCI


17. T. F. Lien, W. Chen, Y. L. Hsu, H. L. Chen, R. Y.-Y. Chiou (2006) Influence of Soy Aglycone Isoflavones on Bone-related Traits and Lens Protein Characteristics of Ovariectomized Rats and Bioactivity Performance of Osteoprogenitor Cells, J. Agri. & Food Chem. (Accepted)  SCI


18. Wenlung Chen陳文龍 (2009 ) Structural Studies of the Complex Between Akt-in and the Akt2-PH Domain Suggest that the Peptide Acts as an Allosteric Inhibitor of the Akt Kinase. The Open Spectrosco. J. 3,65-76


19. A. K. H. Chen, R. Y. Y. Lin, E. Z. J. Hsieh, P. H. Tu, R. P. Y. Chen, T. Y. Liao, W. Chen, C.--H. Wa (2010) Induction of Amyloid Fibrils by the C- Terminal Fragments of TDP-43 in Amyotrophic Lateral Sclerosis. J. Am. Chem. Soc. 132,1186-1187


20. P.-C. Shih, G.-C. Li, K.-J. Yang, W. Chen, D.-L. Tzou* (2011) Conformational analysis of steroid hormone molecules in the lipid environment - A solid-state NMR approach. Steroids 76,558-562


21 .G.-C. Li, D-R. Wang, W. Chen, D.-L. M. Tzou* (2012) Solid-state NMR analysis of steroidal conformation of 17α- and 17β-estradiol in the absence and presence of lipid environment. Steroids 77,185-189


22. 陳文G. C.-H Liu, B. P.-W Chen, N. T.-J. Ye, C.-H. Wang, W. Chen, H.-M. Lee, S. I. Chan, J. J.-T. Huan (2013) Delineating the membrane-disrupting and seeding properties of the TDP-43 amyloidogenic core. Chem. Comm. 49, 11212-11214


23. C.-H. Wang, M.-J. Chen, C.-Y. Tseng, K.-M. Wei, W. Chen* (2013) Structural characterization of functional compositions isolate from Dioscorea Purpurea (Cultivar of Ming-Chien) by Raman spectroscopy.  Natural Product and Chemistry Research 1,110


24. D-R. Wang, J-C. Hsiao,C-H. Wong, G-C Li, S-C. Lin, S. S-F. Yu, W. Chen, W. Chang and D-L. M. Tzou* (2014) Vaccinia Viral Protein A27 is Anchored to Interaction with Viral Membrane Protein A17 the Viral Membrane via a Cooperative.  Journal of Biological Chemistry. 289, 6639-6655 


25. C-S. Sun, C. Y- H. Wang, P-W. Chen, R-Y. He, C-H. Liu, C-H. Wang, W. Chen, Y. Chern, J. J.T. Huang (2014) The influence of pathological mutations and proline substitutions in TDP-43 Glycine-rich peptides on its amyloid properties and cellular toxicity. PLOSone 9, e103644


26. 陳文龍(2015) Conformational switch of polyglutamine-expanded huntingtin into benign aggregates leads to neuroprotective effect. Scientific reports 5, 14992 


27. J. S. Jin, L. F. Lin, J. C. Chen, C. C. Huange, J. H. Sheu, W. Chen, T. Y. Tsao, and C. W. Hsu (2015) Increased cyclin T1 expression as a favorable prognostic factor intreating gastric adenocarcinoma. Oncology Letters 10, 3712-3718 


28. C. H. Wang, C.C. Huang, L.L. Lin, and W. Chen* (2016) The Effect of Disulfide Bonds on Protein Folding, Unfolding, and Misfolding Investigated by FT-Raman Spectroscopy. Journal of Raman Spectroscopy 47,00


29. LIEN. F. Lin , JONG. S. Jin , J. C. Chen , C. C. Huang , J. H. Sheu , W. Chen, and C. W. Hsu(2016) Positive cyclin T expression as a favorable prognostic factor in treating gastric gastrointestinal stromal tumors. MOLECULAR AND CLINICAL ONCOLOGY 10, 3892


30. C. W. Hsu, C. C. Huang, J. H. Sheu, C. W Lin, L. F. Lin, J. S Jin, L. K. Chau, W. Chen*(2016) Differentiating gastrointestinal stromal tumors from gastric adenocarcinomas and normal mucosae using confocal Raman microspectroscopy. Journal of Biomedical Optics 21,00


31. C. W. Hsu, C. C. Huang, J. H. Sheu, C. W. Lin, L. F. Lin, J. S. Jan, L. A. Chau, W. Chen*(2016) Novel Method for Differentiating Histological Types of Gastric Adenocarcinoma by Using Confocal Raman Microspectroscopy. PLoS One 0,1-12






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