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20215月份
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最後更新2021/05/14
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::: * 首頁 > 師資陣容new5 > 林榮流教授

* * 林榮流教授
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友善列印

 


林榮流 特聘教授教授近照

一、學歷:

南非開普敦大學微生物學博士

二、經歷:

1991-1995 新竹食品工業研究所研究員

1995-1998 弘光科技大學食品營養系副教授

1998-2002 弘光科技大學食品營養系教授

2002-2004 嘉義大學應用化學系暨研究所副教授

2004-present 嘉義大學應用化學系暨研究所教授

三、專業領域:

生物化學

四、連絡方式( Tel or e-mail):

  052717969;

   llin@mail.ncyu.edu.tw

五、研究方向:

 My research interest has been focused on elucidating the mechanism of action and the structure-function relationship of enzymes. One ongoing project is to develop an effective enzymatic method for synthesizing theanine from glutamine and ethylamine by Escherichia coli γ-glutamyltranspeptidase (γ-GGT). Moreover, the conserved residues of recombinant E. coli γ-GGT have been replaced with other amino acids by site-directed mutagenesis. The preliminary results show that some of the residues are involved in the autocatalytical processing of the enzyme. We also cloned and expressed other γ-GGTs from Bacillus stearothermophilus and B. kaustophilus in recombinant E. coli. The overproduced enzymes have great potential for the bioconversion of medical agents. The specific aims include identifying phylogenetic analysis and biochemical characterization of a thermostable dihydropyrimidinase from Bacillus sp. TS-23, analyzing the functions of the conserved amino acids of microbial leucine aminopeptidases (LAPs), and recovering the industrial important enzymes by adsorption-elution on raw starch. The other ongoing project is to study the structure-function relationship of B. stearothermophilus LAP. To study the catalytic mechanism of this enzyme, B. stearothermophilus LAP is subjected to structural analysis by X-ray crystallography and to functional analysis by mutagenesis. By combing the data from both approaches, we hope that the molecular action of LAP can be appreciated in the near future.

六、期刊著作:校務系統研究成果

1.       Lin MG, Chi MC, Chen YY, Wang TF, Lo HF*, Lin LL* (2016) Site-directed mutagenesis of a conserved Asn450 residue of Bacillus licheniformis γ-glutamyltranspeptidase. International Journal of Biological Macromolecules 91:416-425. 

2.       Wang CH, Huang CC, Lin LL, Chen W* (2016) The effect of disulfide bonds on protein folding, unfolding, and misfolding investigated by FT–Raman spectroscopy. Journal of Raman SpectroscopyDOI: 10.1002/jrs.4935. 

3.       Lo HF, Chen BE, Lin MG, Chi MC, Wang TF*, Lin LL* (2016) Gene expression and molecular characterization of a chaperone protein HtpG from Bacillus licheniformisInternational Journal of Biological Macromolecules 85:179-191. 

4.       Lin MG, Wang TF, Chen YY, Chi MC*, Lin LL* (2016) Effects of organic co-solvents on catalytic activity and structural stability of three different Bacillus enzymes. Journal of the Taiwan Institute of Chemical Engineers 59:126-131. 

5        Lin MG, Chi MC, Naveen V, Li YC, Lin LL*, Hsiao CD* (2016) Bacillus licheniformis trehalose-6-phosphate hydrolase structures suggest keys to substrate specificity. Acta Crystallographica D72:59-70.

6.       Pica A, Chi MC, D’Ischia M, Chen YY, Lin LL, Merlino A* (2016) The maturation mechanism of γ-glutamyltranspeptidases: insights from the crystal structure of a precursor mimic of the enzyme fromBacillus licheniformisBiochimica et Biophysica Acta – Proteins and Proteomics 1864:195-203. 

7.       Chen YY, Lo HF, Wang TF, Lin MG, Lin LL*, Chi MC* (2015) Enzymatic synthesis of γ-L-glutamyl-S-allyl-L-cysteine, a naturally occurring organosulfur compound from garlic, by Bacillus licheniformis γ-glutamyltranspeptidase. Enzyme and Microbial Technology 75-76:18-24.

8         Liao HF*, Wu DR, Dai JL, Chi MC, Lin LL* (2015) Immunomodulatory activity of the polysaccharide-rich extract from edible cyanobacterium Nostoc commune. Medical Science 3(4):112-123.

9.       Chen JH, Chi MC, Lin MG, Lin LL*, Wang TF (2015) Beneficial effect of sugar osmolytes on the refolding of guanidine hydrochloride-denatured trehalose-6-phosphate hydrolase from Bacillus licheniformis.BioMed Research International Article ID 806847. 

10      Lin MG, Chen BE, Chi MC, Wang TF, Lo HF, Lin LL* (2015) Tryptophan substitution at three specific phenylalanine locations of Bacillus licheniformis nucleotide exchange factor GrpE: structural and functional effects. International Journal of Biological Macromolecules 72:724-731. 

11.    Chi MC, Lo YH, Chen YY, Lin LL*, Merlino A* (2014) γ-Glutamyl transpeptidase architecture: effect of extra sequence deletion on  autoprocessing, structure and stability of the protein from Bacillus licheniformisBiochimica et Biophysica Acta – Proteins and Proteomics 1844:2290-2297. 

12.    Lin LL, Chen YY, Chi MC, Merlino A* (2014) Low resolution X-ray structure of γ-glutamyltranspeptidase from Bacillus licheniformis: opened active site cleft and a cluster of acid residues potentially involved in the recognition of a metal ion. Biochimica et Biophysica Acta - Proteins and Proteomics 1844:1523-1529. 

13.    Juang TY*, Kan SJ, Chen YY, Tsai YL, Lin MG, Lin LL* (2014) Preparation of surface-functionized hyperbranched poly(amido acids) magnetic nanocarriers for the covalent immobilization of a bacterialγ-glutamyltranspeptidase. Molecules 19:4997-5012. 

14.    Ong PL, Chuang TT, Wang TF, Lin LL* (2014) Identification of critical amino acid residues for chloride binding of Bacillus licheniformis trehalose-6-phosphate hydrolase. Biologia 69(1):1-9. 

15.    Wang TF, Lin MG, Lo HF, Chi MC, Lin LL* (2014) Biophysical characterization of a recombinant aminopeptidase II from the thermophilic bacterium Bacillus stearothermophilusJournal of Biological Physics 40:25-40. 

16.    Chi MC, Lin MG, Wang TF, Lo HF, Lin LL* (2013) Influence of asparagine mutations on enzymatic activity and thermostability of a recombinant α-amylase from alkaliphilic Bacillus sp. strain TS-23.Journal of Enzyme Research 4:46-49.

17.    Chen YY, Tsai MG, Chi MC, Wang TF*, Lin LL* (2013) Covalent immobilization of Bacillus licheniformis γ-glutamyltranspeptidase on aldehyde-functionalized magnetic nanoparticles. International Journal of Molecular Sciences 14:4613-4628.

18.    Lin LL, Merlino A* (2013) Heterogeneous nucleation helps the search for initial crystallization conditions of γ-glutamyl transpeptidase from Bacillus licheniformisActa Crystallographica F69:669-672. 

19.    Ong PL, Chuang TT, Wang TF, Lin LL*. 2013. Implications of amino acid residues Arg201 and Asn327 on chloride binding of trehalose-6-phosphate hydrolase, TreA, from Bacillus licheniformisBiologia(Under review)

20.    Lo HF*, Chen YZ, Lin LL*. 2013. Simultaneous purification and immobilization of histidine-taggedBacillus licheniformis aldehyde dehydrogenase. Journal of Pure and Applied Microbiology (accepted for publication)

21.    Chen BE, Lin MG, Lo HF, Wang TF, Chi MC*, Lin LL*. 2013. Introduction of a unique tryptophan residue into various positions of Bacillus licheniformis DnaK. International Journal of Biological Macromolecules 52:231-243. 

22.    Chi MC, Chen YY, Lo HF, Lin LL*. 2012. Experimental evidence for the involvement of amino acid residue Glu398 in the autocatalytic processing of Bacillus licheniformis γ-glutamyltranspeptidase. FEBS Open Bio 2:298-304.

23.   Hu HY, Yang JC, Chen JH, Chi MC*, Lin LL*. 2012. Enzymatic characterization of Bacillus licheniformis γ-glutamyltranspeptidase fused to N-terminally truncated forms of Bacillus sp. TS-23 α-amylase. Enzyme and Microbial Technology 50:86-94. 

24.    Lee YC*, Lin DT, Chen HL, Lo HF, Hu HY, NW Hsiao, Lin LL*. 2012. Characterization of glycine substitution mutations within the NAD+-binding site of Bacillus licheniformis aldehyde dehydrogenase.Protein & Peptide Letters 19: 1183-1193. 

25.   Chen YH, Chi MC, Wang TF, Chen JC, Lin LL*. 2012. Preparation of magnetic nanoparticles and their use for immobilization of C-terminally lysine-tagged Bacillus sp. TS-23 α-amylase. Applied Biochemistry and Biotechnology 166:1711-1722. 

26.    Chuang TT, Ong PL, Wang TF, Huang HB, Chi MC, Lin LL*. 2012. Molecular characterization of a novel trehalose-6-phosphate hydrolase, TreA, from Bacillus licheniformisInternational Journal of Biological Macromolecules 50:459-470. 

27.    Chi MC, Wu TJ, Chen HL, Lo HF*, Lin LL*. 2012. Counteraction of temperature- and chemical-induced denaturation of a recombinant Bacillus sp. TS-23 α-amylase by sorbitol. Journal of Industrial Microbiology and Biotechnology 39: 1779-1788. 

28.    Lee YC, Lin DT, Ong PL, Chen HL, Lo HF*, Lin LL*. 2011. Contribution of conserved Glu255 and Cys289 residues to catalytic activity of a recombinant aldehyde dehydrogenase from Bacillus licheniformisBiochemistry-Moscow 76:1233-1241. 

29.    Lo HF, Su JY, Chen HL, Chen JC, Lin LL*. 2011. Biophysical studies of a NAD(P)+-dependent aldehyde dehydrogenase from Bacillus licheniformisEuropean Biophysics Journal 40:1131-1142. 

30.    Hung CP, Yang JC, Chen JH, Chi MC, Lin LL*. 2011. Unfolding analysis of the mature and unprocessed forms of Bacillus licheniformis γ-glutamyltranspeptidase. Journal of Biological Physics 37:463-475.

31.    Chen HL, Lo HF*, Chang CT, Lin LL*. 2011. Substrate specificity and kinetic characterization of a recombinant dipeptidyl carboxypeptidase from Escherichia coli novablue. Research Journal of Microbiology 6(4):410-417

32.    Yang JC, Liang WC, Chen YY, Chi MC, Lo HF, Chen HL, Lin LL*. 2011. Biophysical characterization of Bacillus licheniformis and Escherichia coli γ-glutamyltranspeptidases: a comparative analysis.International Journal of Biological Macromolecules 48:414-422. 

33.    Lin MG, Liang WC, Chen BE, Chou WM, Lin LL*. 2011. Involvement of residues Asp8, Asn13, Glu145, Asp168, and Thr173 in the chaperone activity of a recombinant DnaK from Bacillus licheniformisJournal of Molecular Microbiology and Biotechnology 20:29-42. 

34.    Chi MC, Wu DR, Chuang TT, Chen HL, Lo HF, Lin LL*. 2010. Biophysical characterization of a recombinant α-amylase from thermophilic Bacillus sp.. TS-23. The Protein Journal 29:572-582. (SCI)

35.    Tsai MY, Chang WH, Liang JY, Lin LL, Chang GC, Chang HP*.  2010. Essential covalent linkage between the chymotrypsin-like domain and the extra domain of the SARS-CoV main protease. Journal of Biochemistry (Tokyo) 149(3):349-358. 

36.   Chen YH, Chuang LY, Lo HF, Lin LL*, Chi MC*. 2010. Mutational analysis of the proposed calcium-binding aspartates of a truncated α-amylase fromBacillus sp. strain TS-23. Annals of Microbiology 60(2):307-315. 

37.     Lin MG, Chen BE, Liang WC, Chou WM, Kuo LY, Lin LL*. 2010. Site-saturation mutagenesis of leucine 134 of Bacillus licheniformis nucleotide exchange factor GrpE reveals the importance of this residue to the co-chaperone activity. The Protein Journal 29:365-372. 

38.    Huang CL, Cheng WC, Yang JC, Chi MC, Chen JH, Lin HP, Lin LL*. 2010. Preparation of carboxylated magnetic particles for the efficient immobilization of C-terminally lysine-tagged Bacillus stearothermophilus aminopeptidase II. Journal of Industrial Microbiology and Biotechnology 37:717-725. 

39.    Chi MC, Chen AH, Wu DR, Lo HF, Lin LL*. 2010. Engineering of a truncated α-amylase of Bacillus sp. strain TS-23 for the simultaneous improvement of thermal and oxidative stabilities. Journal of Bioscience and Bioengineering 109:531-538. 

40.    Chang HP, Liang WC, Lyu RC, Chi MC, Wang ZF, Su KL, Hung HC, Lin LL*. 2010. Effects of C-terminal truncation on the autocatalytic processing of a recombinant γ-glutamyltranspeptidase from Bacillus licheniformis. Biochemistry-Moscow 75(7):919-929. 

41.    Chi MC, Chang HP, Chang GG, Wang TF, Huang HB, Lin LL*. 2010. Biophysical properties of a recombinant leucyl aminopeptidase from Bacillus kaustophilusBiochemistry-Moscow 75(5):642-647. 

42.    Liang WC, Lin MG, Chi MC, Chou WM, Chang HP, Lin LL*. 2009. Residues Leu52 and Leu134 are important for the structural integrity of a nucleotide exchange factor GrpE from Bacillus licheniformisInternational Journal of Biological Macromolecules 45:352-358. 

43.    Lyu RC, Hu HY, Kuo LY, Lo HF, Ong PL, Chang HP, Lin LL*. 2009. Role of the conserved Thr399 and Thr417 residues of a recombinant Bacillus licheniformis γ-glutamyltranspeptidase as evaluated by mutational analysis. Current Microbiology 59:101-106. 

44.     Liang WC, Lin MG, Hu HY, Lo HF, Chang HP, Lin LL*. 2009. Deletion analysis of the C-terminal region of a molecular chaperone DnaK from Bacillus licheniformisArchives of Microbiology 191:583-593. (SCI)

45.    Lo HF, Hu HY, Hung CP, Chen SC, Lin LL*. 2009. Cobalt-chelated magnetic particles for one-step purification and immobilization of His6-tagged Escherichia coli γ-glutamyltranspeptidase. Biocatalysis & Biotransfomation 27 (5-6):318-327. 

46.    Lo CK, Kao CH, Wang WC, Wu HM, Hsu WH, Lin LL*, Hu HY*. 2009. Engineering of the critical residues at the stereochemistry-gate loops of Brevibacillus agri dihydropyrimidinase for the production of L-homophenylaalnine. Process Biochemistry 44:309-315. 

47.    Chen HL, Chang CT, Lin LL, Li TY, Lo HF*. 2009. The dipeptidyl carboxypeptidase of Escherichia coli novablue: overproduction and molecular characterization of the recombinant enzyme. World Journal of Microbiology and Biotechnology 25:323-330. 

48.    Liang WC, Wang XH, Lin MG, Chang HP, Lin LL*. 2009. A 70-kDa molecular chaperone, DnaK, from the industrial bacterium Bacillus licheniformis: gene cloning, purification, and molecular characterization of the recombinant protein. Indian Journal of Microbiology 49:151-160. 

49.    Hsu WH, Ong PL, Chen SC, Lin LL*. 2009. Contribution of Ser463 residue to the enzymatic and processing activities of Escherichia coli g-glutamyltranspeptidase. Indian Journal of Biochemistry & Biophysics 46:281-288. 

50.   Chi MC, Ong PL, Hsu WH, Chen YH, Huang HB*, Lin LL*. 2008. Role of the invariant Asn345 and Asn435 residues in a leucine aminopeptidase from Bacillus kaustophilus as evaluated by site-directed mutagenesis. International Journal of Biological Macromolecules 43:481-487. 

51.    Lin LL*, Yang LY, Hu HY, Lo HF. 2008. Influence of N-terminal truncations on the functional expression of Bacillus licheniformis γ-glutamyltranspeptidase in recombinant Escherichia coliCurrent Microbiology 57:603-608. 

52.    Lin LL*, Chen YP, Yang JC, Hua YW, Wang WC, Kuo LY. 2008. Significance of the conserved Tyr352 and Asp380 residues in the catalytic activity of Bacillus stearothermophilus aminopeptidase II as evaluated by site-directed mutagenesis.  The Protein Journal 27:215-222. 

53.    Lin LL, Huang CC, Lo HF*. 2008. Impact of Arg210-Ser211 deletion on thermostability of a truncatedBacillus sp. strain TS-23 α-amylase. Process Biochemistry 43:559-565. 

54.    Lo HF, Chou WM, Chen PJ, Lin LL*. 2008. Influence of signal-peptide truncations on the functional expression of Escherichia coli novablue γ-glutamyltranspeptidase. Journal of Basic Microbiology 48: 260-268. 

55.     Hung CP, Lo HF, Hsu WH, Chen SC, Lin LL*. 2008. Immobilization of Escherichia coli Novablue γ-glutamyltranspeptidase in Ca-alginate-k-carrageenan beads. Applied Biochemistry and Biotechnology150: 157-170. 

56.    Wu CL, Chen YP, Lo HF, Lin LL*. 2008. Characterization of lysine-tagged Bacillus stearothermophilus leucine aminopeptidase II immobilized onto carboxylated gold nanoparticles. Journal of Molecular Catalysis B Enzymatic 54:83-89. 

57.    Chi MC, Liu JS, Wang WC, Lin LL*, Huang HB*. 2008. Site-directed mutagenesis of the conserved Ala348 and Gly350 residues at the putative active site of Bacillus kaustophilus leucine aminopeptidase. Biochimie 90:811-819. 

58.    Ong PL, Yao YF, Weng YM, Hsu WH, Lin LL*. 2008. Residues Arg114 and Arg337 are critical for the proper function of Escherichia coli γ-glutamyltranspeptidase. Biochemical and Biophysical Research Communications 366:294-300. 

59.     Lin LL, Chen SH, Huang CC, Lo HF*. 2008. Glutamic acid 219 is critical for the thermostability of a truncated α-amylase from alkaliphilic Bacillus sp. strain TS-23. World Journal of Microbiology and Biotechnology 24:619-626.

60.    Chi MC, Lu RQ, Lin LL*, Huang HB*. 2008. Characterization of Bacillus kaustophilus leucine aminopeptidase immobilized in Ca alginate/k-carrageenan beads. Biochemical Engineering Journal 39: 376-382. 

61.    Lo HF, Lin LL*, Chen PJ, Chou WM*. 2007. Site-directed mutagenesis of the conserved Thr407, Asp433, and Met464 residues in the small subunit of Escherichia coli γ-glutamyltranspeptidase. Indian Journal of Biochemistry & Biophysics 44:197-203. 

62.    Lin LL, Chen MH, Chien HR, Kan SC, Hu HY*, Hsu WH*. 2007. Characterization of a bifunctional aminoacylase/carboxypeptidase from radioresistant bacterium Deinococcus radiodurans R1. Journal of Biotechnology 128: 322-334. 

63.    Yao YF, Weng YM, Hu HY, Ku KL, Lin LL*. 2006. Expression optimization and biochemical characterization of a recombinant γ-glutamyltranspeptidase from Escherichia coli Novablue. TheProtein Journal 25: 431-441. 

64.    Yang HL, Chen RS, Chen W, Lin LL*. 2006. Identification of glutamate residues important for catalytic activity of Bacillus stearothermophilus leucine aminopeptidase II. Antonie van Leeuwenhoek 90: 195-199. 

65.     Lin LL*, Chou PR, Hua YW, Hsu WH. 2006. Overexpression, one-step purification, biochemical characterization of a recombinant γ-glutamyltranspeptidase from Bacillus licheniformisApplied Microbiology and Biotechnology 73: 103-112. 

66.    Lin LL, Chen PJ, Liu JS, Wang WC, Lo HF.* 2006. Identification of glutamate residues important for catalytic activity or thermostability of a truncated Bacillus sp. strain TS-23 α-amylase by site-directed mutagenesis. The Protein Journal 25: 232-239. 

67.    Chi MC, Huang HB, Liu JS, Wang WC, Liang WC, Lin LL*. 2006. Residues threonine 346 and leucine 352 are critical for the proper function of Bacillus kaustophilus leucine aminopeptidase. FEMS Microbiology Letters 260: 156-161. 

68.    Yao YF, Hu HY, Lin LL.* 2006. Over-expression of a recombinant γ-glutamyltranspeptidase from Escherichia coliIndian Journal of Biochemistry and Biophysics 43: 345-350. 

69.    Huang HB, Chi MC, Hsu WH, Liang WC, Lin LL.* 2005. Construction and one-step purification of Bacillus kaustophilus leucine aminopeptidase fused to the starch-binding domain of Bacillus sp. strain TS-23 α-amylase. Bioprocess and Biosystems Engineering 27: 389-398. 

70.    Lin LL, Hsu WH, Hsu WY, Kan SC, Hu HY.* 2005. Phylogenetic analysis and biochemical characterization of a thermostable dihydropyrimidinase from alkaliphilic Bacillus sp. TS-23. Antonie van Leeuwenhoek88: 189-197. 

71.    Chen RS, Yang SL, Hua YW, Chi MC, Lin LL.* 2005. Directed mutagenesis of the conserved asparagines residues of Bacillus stearothermophilus leucine aminopeptidase II. World Journal of Microbiology and Biotechnology 21: 1477-1482. 

72.    Hwang GY, Kuo LY, Tsai MR, Yang SL, Lin LL.* 2005. Histidines 345 and 378 of Bacillus stearothermophilus leucine aminopeptidase II are essential for the catalytic activity of the enzyme. Antonie van Leeuwenhoek 87: 355-359. 

73.    Hua YW, Chi MC, Lo HF, Ku LY, Lin LL.* 2005. Adsorption-elution purification of the chimeric Bacillus stearothermophilus leucine aminopeptidase II with raw-starch-binding activity. World Journal of Microbiology and Biotechnology 21: 689-694. 

74.    Lo HF, Chen YH, Hsiao NM, Chen HL, Hu HY, Hsu WH, Lin LL.* 2005. Stabilization of a truncated Bacillussp. strain TS-23 α-amylase by replacing histidine 436 with aspartate. World Journal of Microbiology and Biotechnology 21: 411-416. 

75.    Liao YH, Wang CH, Tseng CY, Chen HL, Lin LL, Chen W.* 2004. Compositional and conformational analysis of yam proteins by near infrared Fourier Transform Raman spectroscopy. Journal of Agricultural and Food Chemistry 52: 8190-8196. 

76.    Chi MC, Chou WM, Wang CH, Chen W, Hsu WH, Lin LL.* 2004. Generating oxidation-resistant variants of Bacillus kaustophilus leucine aminopeptidase by substitution of the critical methionine residues with leucine. Antonie van Leeuwenhoek 86: 355-362 

77.    Chi MC, Chou WM, Hsu WH, Lin LL.* 2004. Identification of amino acid residues essential for catalytic reaction of Bacillus kaustophilus leucine aminopeptidase. Bioscience, Biotechnology and Biochemistry 68:1794-1797. 

78.    Hua YW, Chi MC, Lo HF, Hsu WH, Lin LL.* 2004. Fusion of Bacillus stearothermophilus leucine aminopeptidase II with the raw starch-binding domain of Bacillus sp strain TS-23 α-amylase generates a chimeric enzyme with the thermostability and catalytic efficiency. Journal of Industrial Microbiology and Biotechnology 31: 273-277. 

79.     Kuo LY, Hwang GY, Yang SL, Chen RH, Chen W, Lin LL.* 2004. Inactivation of Bacillus stearothermophilus leucine aminopeptidase II by hydrogen peroxide and site-directed mutagenesis of the methionine residues on the enzyme. The Protein Journal 23: 295-302. 

80.    Hsu SK, Lin LL, Lo HH, Hsu WH.* 2004. Mutational analysis of feedback inhibition and catalytic sites of prephenate dehydratase from Corynebacterium glutamicumArchives of Microbiology 181: 237-244. 

81.    Lai EYC, Chyau CC, Mau JL, Chen CC, Lai YJ, Shih CF, Lin LL.* 2004. Antimicrobial activity and cytotoxicity of the essential oil of Curcuma zedoaria. American Journal of Chinese Medicine 32: 281-290. 

82.    Lin LL, Wu CP, Chi MC, Chou WM, Hu HY, Hsu WH.* 2004. A thermostable leucine aminopeptidase from Bacillus kaustophilus CCRC 11223. Extremophiles 8: 79-87. 

83.    Lo FH, Chiang WY, Chi MC, Hu HY, Lin LL.* 2004. Site-directed mutagenesis of the conservedthreonine, tryptophan, and arginine residues in the raw starch-binding domain of Bacillus sp. strain TS-23 α-amylase. Current Microbiology 48: 280-284. 

84.    Chang CT, Lo HF, Chi MC, Hsu WH, Lin LL.* 2003. Identification of essential histidine residues in a recombinant α-amylase of thermophilic and alkaliphilic Bacillus sp. strain TS-23. Extremophiles 7: 505-509. 

85.    Lin LL*, Lo HF, Chi MC, Ku KL. 2003. Functional expression of the raw starch-binding domain of Bacillussp. strain TS-23 α-amylase in recombinant Escherichia coliStarch/Stärke 55:197-202. 

86.    Kuo LY, Hwang GY, Lai YJ, Yang SL, Lin LL.* 2003. Overexpression, purification, and characterization of the recombinant leucine aminopeptidase II of Bacillus stearothermophilusCurrent Microbiology 47: 40-45. 

87.    Lin LL*, Lo HF, Chiang WY, Hu HY, Hsu WH, Chang CT. 2003. Replacement of methionine 208 in a truncated Bacillus sp. TS-23 α-amylase with oxidation-resistant leucine enhances its resistance to hydrogen peroxide. Current Microbiology 46: 211-216. 

88.    Lo FH, Lin LL*, Chiang WY, Chi MC, Hsu WH, Chang CT. 2002. Deletion analysis of the C-terminal region of the α-amylase of Bacillus sp. strain TS-23. Archives of Microbiology 178: 115-123. 

89.    Lin LL*, Lo HF, Chen JN, Ku KL, Hsu WH. 2002. Isolation of a recombinant Bacillus sp. TS-23 α-amylase by adsorption-elution on raw starch. Starch/Stärke 54: 338-342. 

90.    Chien HR, Lin LL, Chao SH, Chen CC, Wang WC, Shaw CY, Tsai YC, Hsu WH.* 2002. Purification, characterization, and genetic analysis of a leucine aminopeptidase from Aspergillus sojae. Biochimica et Biophysica Acta 1576: 119-126. 

91.   Lo FH, Lin LL*, Li CC, Hsu WH, Chang CT. 2001. The N-terminal signal sequence and the last 98 amino acids are not essential for the secretion of Bacillus sp. TS-23 α-amylase in Escherichia coliCurrent Microbiology 43: 170-175. 

92.    Lin LL, Chien HR, Liao FH, Hsu WH*. 2001. Identification of essential cysteine residues of Corynebacterium glutamicum 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase. Current Microbiology 42: 426-431. 

93.    Lo FH, Lin LL, Chen SL, Hsu WH, Chang CT.* 2001. Enzymatic properties of a SDS-resistant Bacillus sp. TS-23 α-amylase produced by recombinant Escherichia coliProcess Biochemistry 36: 743-750. 

94.    Liao FH, Lin LL, Chien HR, Hsu WH.* 2001. Serine 187 is a crucial residue for allosteric regulation of Corynebacterium glutamicum 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase. FEMS Microbiology Letters 194: 59-64. 

95.    Lin LL, Chien HR, Wang WC, Hwang TS, Fu HM, Hsu WH.* 2000. Overexpression of Trigonopsisvariabilis D-amino acid oxidase gene and characterization of its inactive mutants. Enzyme and Microbial Technology 27:482-491. 

96.    Ma YJ, Lin LL, Chien HR, Hsu WH.* 2000. Efficient utilization of starch by a recombinant strain of Saccharomyces cerevisiae producing glucoamylase and isoamylase. Biotechnology and Applied Biochemistry 31:55-59. 

97.    Hwang TS, Fu HM, Lin LL, Hsu WH.* 2000. High-level expression of Trigonopsis variabilis D-amino acid oxidase in Escherichia coli using lactose as inducer. Biotechnology Letters 22:655-658. 

98.    Ju SS, Lin LL, Chien HR, Hsu WH.* 2000. Substitution of the critical methionine residues in Trigonopsis variabilis D-amino acid oxidase with leucine enhances its resistance to hydrogen peroxide. FEMS Microbiology Letters 186:215-219. 

99.    Lin LL, Wang WC, Ju SS, Chien HR, Hsu WH*. 1999. The role of a conserved histidine residue, His-324, in Trigonopsis variabilis D-amino acid oxidase. FEMS Microbiology Letters 176:443-448 

100. Chou CC*, Lin LL, Chung KT. 1999. Antimicrobial activity of tea as affected by the degree of fermentation and manufacturing season. International Journal of Food Microbiology 48:125-130. 

101.  Ju SS, Lin LL, Wang WC, Hsu WH*. 1998. A conserved aspartate is essential for FAD binding and catalysis in the D-amino acid oxidase from Trigonopsis variabilisFEBS Letters 436:119-122. 

102. Chen PH, Lin LL, Hsu WH*. 1998. Expression of Pseudomonas amyloderamosa isoamylase gene in Saccharomyces cerevisiaeBiotechnology Letters 20:735-739. 

103. Lin LL, Ma YJ, Chien HR, Hsu WH*. 1998. Construction of an amylolytic yeast by multiple integration of an Aspergillus awamori glucoamylase gene into Saccharomyces cerevisiae chromosome. Enzyme and Microbial Technology 23:360-365. 

104. Lin LL, Chyan CC, Hsu WH*. 1998. Production and properties of a raw-starch-degrading amylase from the thermophilic and alkaliphilic Bacillus sp. TS-23. Biotechnology and Applied Biochemistry 28:61-68. 

105. Lin LL, Hsu WH*. 1997. Lactose-induced expression of Bacillus sp. TS-23 amylase gene in Escherichia coli regulated by a T7 promoter. Letters in Applied Microbiology 24:365-368. 

106. Lin LL*, Hsu WH, Chu WS. 1997. A gene encoding for an α-amylase from thermophilic Bacillus sp. TS-23 and its expression in  Escherichia coliJournal of Applied Microbiology 82:325-334. 

107. Lin LL*, Tsau MR, Chu WS. 1996. Purification and properties of a 140 kDa amylopullulanase from thermophilic and alkalophilic Bacillus sp. strain TS-23. Biotechnology and Applied Biochemistry 24:101-107 

108. Lin LL*, Hsu WH. 1995. Expression of Pseudomonas amyloderamosa isoamylase gene in Escherichia coli using an inducible phage T7 expression system. Process Biochemistry 30: 547-552. 

109. Lin LL*, Fang TY, Chu WS, Hsu WH. 1994. Improved elution of isoamylase adsorbed on raw starch and the preservation of purified enzyme. Letters in Applied Microbiology 19: 383-385. 

110. Lin LL*, Tsau MR, Chu WS. 1994. General characteristics of thermostable pullulanases and amylases from an alkalophilic Bacillus sp. TS-23. Applied Microbiology and Biotechnology 42: 51-56. 

111. Wu DH, Wen CY, Lin LL, Chu WS, Hsu WH.* 1994. Effect of pH on isoamylase production by Pseudomonas amyloderamosa WU5315. Letters in Applied Microbiology 19: 67-69. 

112. Fang TY, Lin LL, Hsu WH.* 1994. Recovery of isoamylase from Pseudomonas amyloderamosa by adsorption-elution on raw starch. Enzyme and Microbial Technology 16: 247-252. 

113.  Wu DH, Wen CY, Chu WS, Lin LL, Hsu WH.* 1993. Selection of antibiotic-resistant mutants with enhanced isoamylase activity in Pseudomonas amyloderamosaBiotechnology Letters 15: 883-888. 

114. Tsao LS, Lin LL, Chen JC, Chen JH, Hsu WH.* 1993. Cloning and characterization of an α-amylase gene from Streptomyces lividansBiochimica et Biophysica Acta 1171: 255-262. 

115. Lin LL, Thomson JA.* 1991. An analysis of the extracellular xylanases and cellulases of Butyrivibrio fibriosolvensFEMS Microbiology Letters 84: 197-204. 

116. Lin LL, Thomson JA. 1991. Cloning, sequencing and expression of a gene encoding a 73 kDa xylanase enzyme from the rumen anaerobe Butyrivibrio fibrisolvens H17c. Molecular and General Genetics 228: 55-61. 

117. Lin LL, Rumbak E, Zappe H, Thomson JA, Woods DR.* 1990. Cloning, sequencing and analysis of a Butyrivibrio fibriosolvens gene encoding a β-glucosidase. Journal of General Microbiology 136: 1567-1576. 

118. Wang TT, Lin LL, Hsu WH. 1989. Cloning and expression of a Schwanniomyces occidentalis α-amylase gene in Saccharomyces cerevisiaeApplied and Environmental Microbiology 55: 3167-3172. 

 


七、 研討會論文  

 

1.       Chi MC, Chen YY, Lin LL. 2013. Glutamate 398 is crucial for the autocatalytic processing of Bacillus licheniformis  γ-glutamyltranspeptidase. International Conference on Life Science & Biological Engineering (LS&BE 2013), Tokyo, Japan.

2.       Chen BE, Wu DJ, Lin LL. 2013. Biochemical characteristics and pH-induced conformational changes of high temperature protein G (HtpG/Hsp90) from Bacillus licheniformis. International Conference on Life Science & Biological Engineering (LS&BE 2013), Tokyo, Japan.

3.       Wu DJ, Lin LL, Dai JL, Liao HF. 2013 Bioactivities and immunomodulatory activity of Nostoc commune. International Conference on Life Science & Biological Engineering (LS&BE 2013) , Tokyo, Japan.

4.       Lin DT, Lee YC, Lo HF, Lin LL. 2012. Exploration of the glycine residues essential for NAD+-binding of Bacillus licheniformis aldehyde dehydrogenase. Abstracts of 3rd International Conference on Food Engineering and Biotechnology, Kuala Lumpur, Malaysia.

5.       Chi MC, Lin LL. 2012. Influence of osmolytes on the structure and enzymatic activity of a recombinant α-amylase from Bacillus sp. TS-23. Abstracts of 12th Annual Meeting of the protein Science Society of Japan, Nagoya, Japan.

6.       Chuang ZT, Ong PL, Lin LL. 2012. Biochemical and biophysical characterization of a trahalose-6-phosphate hydrolase form Bacillus licheniformis. Abstracts of 12th Annual Meeting of the protein Science Society of Japan, Nagoya, Japan.

7.       Lin LL, Su JY. 2011. Biophysical characterization of a (NAD(P)+-dependent aldehyde dehydrogenase from Bacillus licheniformis. Abstracts (P03.82) of 36th FEBS Congress, Torino, Italy.

8.       Chi MC, Wu DJ, Lin LL. 2011. Biophysical studies of a truncated α-amylase from thermophilic Bacillus sp. strain TS-23. Abstracts (1016) of IX European Symposium Protein of the Protein Society, Stockholm, Sweden.

9.       Lo HF, Chen HL, Lin LL. 2011. Immobilization of C-terminally lysine-tagged α-amylase onto adipic acid-modified magnetic nanoparticles. Abstracts of the 12th ASEAN Food Conference, Bangkok, Thailand.

10.   Chi MC, Chen YH, Lin LL. 2010. Engineering of Bacillus sp. strain TS-23 α-amylase for improving its thermal and oxidative stabilities. Abstracts (E5.13) of 35th FEBS Congress, Gothenburg, Sweden.

11.   Lin LL, Lin MG, Chen BE, Liang WC, Chi MC. 2010. Importance of leucine 134 to the co-chaperone activity of Bacillus licheniformis nucleotide exchange factor GrpE. Abstracts (E4.06) of 35th FEBS Congress, Gothenburg, Sweden.  

12.   Lin LL, Lin MG, Liang WC, Chi MC. 2009. Role of the C-terminal region of a recombinant DnaK from Bacillus licheniformis as evaluated by a serial deletion. Abstracts of 34th FEBS Congress, Prague, Czech Republic.

13.   Chi MC, Liang WC, Lin LL, Huang HB. 2009. Characterization and C-terminally truncational analysis of a 70-kDa molecular chaperone, DnaK, from the industrial bacterium Bacillus licheniformis. Abstracts of Experimental Biology 2009, New Orleans, Louisiana, USA.

14.   Lin LL, Wu CL, Yang JC, Chen YP, Lo HF. 2008. Characterization of lysine-tagged Bacillus stearothermophilus leucine aminopeptidase II immobilized onto carboxylated gold nanoparticles. Abstracts of 33rd FEBS Congress and 11th IUBMB Conference, Athens, Greece.

15.   Chi MC, Huang HB, Lin LL. 2008. Significance of the conserved Asn345 and Asn435 residues in the catalytic activity of Bacillus kaustophilus leucine aminopeptidase as evaluated by site-directed mutagenesis. Abstracts of 33rd FEBS Congress and 11th IUBMB Conference, Athens, Greece.  

16.   Lin LL, Chen SH, Huang CC, Lo HF. 2007. Glutamic acid 219 of a truncated α-amylase from alkaliphilic and thermophilic Bacillus sp. strain TS-23 plays a critical role for its thermostability. Abstracts of Annual Scientific Meeting & Exhibition of the Australian Society for Microbiology, Adelaide, Australia.

17.   Chen PJ, Chou WM, Lo HF, Lin LL. 2007. Residues Asn428 and Asn452 are important for the catalytic activity of Escherichia coli novablue g-glutamyltranspeptidase. Abstracts of Annual Scientific Meeting & Exhibition of the Australian Society for Microbiology, Adelaide, Australia.

18.   Chi MC, Lin LL, Huang HB. 2007. Residues Ala348 and Gly350 are critical for the catalytic activity of Bacillus kaustophilus leucine aminopeptidase. Abstracts of Annual Scientific Meeting & Exhibition of the Australian Society for Microbiology, Adelaide, Australia.

19.   Lin LL, Lia YH, Wang CH, Chen HL, Chen W. 2004. FT-Raman spectroscopic study of yam protein conformation. Abstracts of the 9th International Conference on Raman Spectroscopy, Gold Coast, Australia.

20.   Lin LL, Chyau CC, Mao JL. 1998. Production and analysis of amylolytic enzymes from thermophilic and alkaliphilic Bacilus sp. TS-23. Abstracts of the IFT Annual Meeting, Atlanta, USA.

21.   Lin LL, Chyau CC, Mao JL. 1997. Cloning, sequencing and expression of an α-amylase gene from thermophilic and alkaliphilic Bacillus sp. TS-23. Abstracts of the IFT Annual Meeting, Florida, USA.

22.   Tsau MR, Lin LL, Chu WS. 1995. A thermostable pullulanase from alkalophilic Bacillus sp. TS-23. Abstracts of the 7th European Congress on Biotechnology, Nice, France.

23.   Fang TY, Chu WS, Lin LL, Hsu WH. 1993. Recovery of isoamylase from Pseudomonas amyloderamosa by raw starch adsorption. Abstracts of the 6th European Congress on Biotechnology, Firenze, Italy.

24.   Lin LL, Rumbak E, Zappe H, Thomson JA, Woods DR. 1991. Cloning, sequencing and analysis of a Butyrivibrio fibrisolvens gene encoding a β-glucosidase. Abstracts of the 6th Binary Symposium on Applied Microbiology, Stellenbosh, South Africa.

25.   Tsao LS, Chen JC, Lin LL, Lee JC, Hsu WH. 1990. Cloning and characterization of theα-amylase from Streptomyces lividans TK-24. Abstracts of ASM Annual Meeting, Anaheim, California, USA.

26.   Wang TT, Lin LL, Hsu WH. 1989. Cloning and expression of a Schwanniomyces occidentalis α-amylase gene in Saccharomyces cerevisiae. Abstracts of the International Conference on Biotechnology, Beijing, China.

 

八、研究計畫:

 

補助

機關

1.

87 融合蛋白質回收系統之建立 (1/3)

國科會

2.

88 融合蛋白質回收系統之建立 (2/3)

國科會

3.

89 融合蛋白質回收系統之建立 (3/3)

國科會

4.

89 嗜高溫及鹼性 Bacillus sp. TS-23 α-amylase 之突變分析

國科會

5 .

90 嗜高溫及鹼性 Bacillus sp. TS-23 α-amylase C- 端區域功能性

國科會

6 .

91  Bacillus sp. TS-23 α-amylase 必需組胺酸之鑑定

國科會

7.

91  Bacillus stearothermophilus leucine aminopeptidase之生化特性及突變分析

國科會

8.

92  Bacillus sp. TS-23 α-amylase 生澱粉結合區之選位突變

國科會

9.

92 改造 Agrobacterium tumefaciens  g -胺基轉胜太以生產茶胺酸

農委會

10.

93 熱穩定白胺酸之酵素特性與突變分析 (1/3)

國科會

11.

94 熱穩定白胺酸之酵素特性與突變分析 (2/3)

國科會

12

95 熱穩定白胺酸之酵素特性與突變分析 (3/3)

國科會

13

95大腸桿菌g-麩基轉胜之基因表現、生物轉換應用及突變分析 (1/3)

國科會

14

96大腸桿菌g麩基轉胜之基因表現、生物轉換應用及突變分析 (2/3)

國科會

15

97大腸桿菌g麩基轉胜之基因表現、生物轉換應用及突變分析 (3/3)

國科會

16

97  Bacillus sp. strain TS-23 α-澱粉之熱穩定性改善及單一步驟磁性粒子純化與其配合啤酒酵母生產酒精 (1/3)

國科會

17

98  Bacillus sp. strain TS-23 α-澱粉之熱穩定性改善及單一步驟磁性粒子純化與其配合啤酒酵母生產酒精 (2/3)

國科會

18

99  Bacillus sp. strain TS-23 α-澱粉之熱穩定性改善及單一步驟磁性粒子純化與其配合啤酒酵母生產酒精 (3/3)

國科會

九、其他:

  1. Fang, T.Y., L.L. Lin , W.S. Chu, and W.H. Hsu. Raw starch adsorption-elution purification of isoamylase. Chinese patent 070723.
  2. Fang, T.Y., L.L. Lin , W.S. Chu, and W.H. Hsu. Method for recovery and purification of isoamylase by adsorption on raw starch. US patent 5811277.

十、教授課目:

生物高分子、核酸化學、生物技術及高等生物化學



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