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National Chiayi University
:::

Hsi-Tien Wu, Professor


Hsi-Tien, Wu Ph.D. 


(吳希天)

 Professor 

Laboratory of Animal Molecular Genetics
Department of BioAgricultural Sciences
National ChiaYi University
TAIWAN

Tel: +886-5-2777767

Fax: +886-5-2717755

E-mail: htwu@mail.ncyu.edu.tw                                                                               


EDUCATION

2004, Ph.D. Animal Science, National Chung Hsing University, Taiwan   
1991, M.S. Animal Science, National Chung Hsing University, Taiwan   


EXPERIENCE


2013/8-present:
Associate professor in Department of BioAgricultural Sciences, National ChiaYi University

2015/8-2018/7:
Chairman of Department of BioAgricultural Sciences, National ChiaYi University

2005/8-2013/7:
Assistant professor in Department of BioAgricultural Sciences (former: Department of Intensive Agriculture), National ChiaYi University

1991/10-2005/7: 
Assistant researcher, superintendent in transgenic core facility and vice- superintendent in research group of National Laboratory Animal Center, National Applied Research Laboratories 
Research title: Molecular Genetics of Laboratory Animal (mice/rats).
Research techniques: Laboratory animal breeding, genetic monitoring, transgenic research, embryo cryopreservation, stem cell culture.


SPECIALTY

1. Molecular Biology
    Gene cloning/expression

2. Embryo/Sperm Cryopreservation
    Mouse embryo/sperm cryopreservation, IVF, ICSI 

3. Transgenic Research
    Transgenic mouse, stem cell research, nuclear transplantation

4. Laboratory Animal Science
    Laboratory animal genetics/breeding/management


RESEARCH PROJECTS

2014/8- 2017/7, National Science Council research project  
Studying the pathophysiologic mechanisms of renin-angiotensin system in liver fibrosis using Ace 2 knockout mouse model
Principle investigator 

2008/8- 2011/7, National Science Council research project  
Studies of metalloproteinase 9 (MMP-9) promoter gene regulation and arrangement of transgenic mouse animal model carried MMP-9 promoter gene for drugs screening of traumatic cardiovascular diseases
Principle investigator 

2007/8- 2008/7, National Science Council research project  
Studies of drugs screening and gene functions monitoring on cardiovascular diseases by transgenic mouse model with matrix metalloproteinase 9 promoter gene
Principle investigator 

2006/8- 2007/7, National Science Council research project  
Studies of drug screening and gene functions monitoring on cardiovascular diseases by transgenic mouse model (II): Cloning and characterization of human and murine matrix metalloproteinase 9 gene promoter
Principle investigator 

2005/8- 2006/7, National Science Council research project  
Studies of three-dimensionally heart model by transgenic mouse for drug screening and gene functions monitoring on cardiovascular diseases (I): Generation of green fluorescent protein gene Pgk-1/GFP transgenic mice and establishment of discontinuous fluorescent heart atlas layout
Principle investigator 

2004/09-2005/08, National Laboratory Animal Center research project   
Cryopreservation of germ cell (embryo/sperm) of Inbred Strain Foundation Colony (I).
Principle investigator

2003/08-2004/12, National Laboratory Animal Center research project   
Recovery of Inbred Strain Colony by Embryo Rederivation.
Principle investigator

2004/01-2004/12, National Laboratory Animal Center research project   
Inbreeding and Cryopreservation of Human Serum Albumin (HSA) Transgenic Mice.
Principle investigator

2003/01-2003/06, National Laboratory Animal Center research project   
Setup of Cryopreservation System of Inbreed Strain Mice.
Principle investigator

2001/09-2002/8, National Science Council research project       
Establishment of National Gene Manipulating Service Center.
Co-principle investigator 


PUBLICATIONS

Refereed papers: 

  • Hung‐Chiuan Chen, Suresh Awale, Chean‐Ping Wu, Hu‐Hui Lee, Hsi‐Tien Wu. 2020. Co‐cultured bone marrow mesenchymal stem cells repair thioacetamide‐induced hepatocyte damage. Cell Biology International. 44(12): 2459-2472. (SCI) 
  • Wu HT, Chuang YW, Huang CP, Chang MH. 2018. Loss of angiotensin converting enzyme II (ACE2) accelerates the development of liver injury induced by thioacetamide. Experimental Animals. 67(1): 41-49. (SCI)
  • Chiu-Mei Kuo, Tsung-Hsien Lin, Yi-Chun Yang, Wen-Xin Zhang, Jinn-Tsyy Lai, Hsi-Tien Wu, Jo-Shu Chang, Chih-Sheng Lin. 2017. Ability of an alkali-tolerant mutant strain of the microalga Chlorella sp. AT1 to capture carbon dioxide for increasing carbon dioxide utilization efficiency. Bioresource Technology. 244(Part 1): 243-251. (SCI)
  • C. M. Kuo, Y. B. Chang, T. H. Lin, Y. C. Yang, W. X. Zhang, J. T. Lai, H. T. Wu, C. S. Lin. 2017. Effects of a Protein-Enriched Crude Lysate from Chlorella Sp. Against Acetaminophen-Induced Liver Injury. IOSR Journal of Biotechnology and Biochemistry. 3(1):82-88.
  • Y. L. Sun, H. T. Wu, C. S. Lin. 2016. Direct colorimetric detection of canine distemper virus by RT-PCR combined with gold nanoparticles. IOSR Journal of Biotechnology and Biochemistry. 2 (6 Part II): 69-74. 
  • Chung-Shen Wu, Wei-Tin Kuo, Chia-Yu Chang, Jun-Yi Kuo, Yi-Ting Tsai, Su-May Yu, Hsi-Ten Wu, Peng-Wen Chen. 2014. The modified rice αAmy8 promoter confers high-level foreign gene expression in a novel hypoxia-inducible expression system in transgenic rice seedlings. Plant Molecular Biology. 85:147-161. (SCI)
  • Cheng-Chung Lin, Hsi-Tien Wu. 2014. Review of the Electroporation Lysis Techniques of Cell. Hsin Hsin Quarterly. 42(1):86-97. (in Chinese)
  • Kai-Jie Yang, Siao-Cian Pan, Yu-Chia Liu, Wei-Ting Lai, and Hsi-Tien Wu. 2013. Establishing a technique for identifying the transgene integration site in mice. Journal of the Agricultural Association of Taiwan. 14(1): 66-85.(in Chinese)
  • Hsi Tien Wu, Syu Sheng Sie, Tang Ching Kuan, Chih Sheng Lin. 2013. Identifying the Regulative Role of NF-kB Binding Sites within Promoter Region of Human Matrix Metalloproteinase 9 (mmp-9) by TNF-α Induction. Applied Biochemistry and Biotechnology. 169 (2):438-449. (SCI)
  • Hui-Shuang Yeh, Ching-Yuan Chen, Yung-Tsai Huang, and Hsi-Tien Wu. 2012. Antioxidant Effect of Vitamin C on Cardiomyoblast H9c2(2-1). Journal of the Agricultural Association of Taiwan. 13:191-203. (in Chinese) 
  • Cheng-Han Huang, Yi-Ming Huang, Yung-Sheng Tseng, Wei-Chi Lee, Jui-Te Wu , Zhi-Jia Zheng, and Hsi-Tien Wu. 2011. Heart dysfunction and fibrosis in rat treated with myocardial ischemia and reperfusion. African Journal of Biotechnology. 10: 16387-16392. (SCI)
  • Y.C. Liu, W.Y. Lin, Y.R. Jhang, S.H. Huang, C.P. Wu, and H.T. Wu. 2011. Efficiency of DNA transfection of rat heart myoblast H9c2(2-1) cells by either polyethyleneimine or electroporation. Applied Biochemistry and Biotechnology.  164: 1172-1182. (SCI) 
  • Wu, H. T., C. K. Chou, Y. C. Hung, and C. K. Yu. 2010. Allotransplantation of Transgenic Mouse Ovaries Expressing Enhanced Green Fluorescent Protein under the Control of the Murine Phosphoglycerate Kinase 1 Promoter. Reproduction in Domestic Animals. 45: 900–906. (SCI) 
  • Pan, H. C., H. T. Wu, F. C. Cheng, C. H. Chen, M. L. Sheu, and C. J. Chen. 2009. Potentiation of angiogenesis and regeneration by G-CSF after sciatic nerve crush injury. Biochemical and Biophysical Research Communications. 382:177-182. (SCI)
  • Wu, H. T., C. K. Chou, and M. C. Huang. 2004. Expression of human serum albumin in milk of transgenic mice using goat β-casein/human serum albumin fusion gene. Asian-Australasian Journal of Animal Sciences. 17: 743-749. (SCI)
  • Wu, H. T., C. K. Chou, C. S. Lin, and M. C. Huang. 2003. Effects of glucose concentration on in vitro fertilization in BALB/c mice. Reproduction in Domestic Animals. 38: 470–474. (SCI)
  • Wu, H. T., C. S., Lin and M. C., Huang. 2003. In vitro and ex vivo green fluorescent protein expression in alveolar mammary epithelial cells and mammary glands driven by the distal 5’ regulative sequence and intron 1 of goat β-casein gene. Reproduction, Fertility and Development. 15:231-239. (SCI)
  • Lin, C. S., C. Y. Liu, H. T. Wu, Y. L. Sun, L. C. Chang, N. T. Yen, P. C. Yang, M. C. Huang and S. J. T. Mao. 1998. SSCP analysis in the D-loop region of porcine mitochondrial DNA as confirmed by sequence diversity. Journal of Animal Breeding and Genetics.115:73-78. (SCI)
  • Lin, C. S., H. T. Wu, C. Y. Liu, Y. L. Sun, L. C. Chang, C. C. Hong, P. C. Yang, S. J. Y. Mao and M. C. Huang. 1997. Amplifying the D-loop Region of Mitochondrial DNA from Whole Blood and Embryonic Cells of Mammalian Animals. Journal of the Agricultural Association of China. 180:80-91. (in Chinese)
  • Hsi-Tien Wu and Mu-Chiou Huang. 1996. Observation of Electroporated Rooster Spermatozoa by Scanning Electron Microscopy. Journal of the Agricultural Association of China. 173:130-139. (in Chinese)
  • H. T. Wu, M. C. Huang. 1995. Effects of electroporation on quality of rooster semen. Asian-Australasian Journal of Animal Sciences. 8:175-179. (SCI)  
  • Chich-Sheng Lin, Chang-Yeu Liu, and Hsi-Tien Wu. 1995. Isolation of mitochondrial DNA from the visceral organs and muscle tissue of pig by sucrose gradient centrifugation. Journal of the Chinese Society of Animal Science. 24:125-138. (in Chinese)
  • Wu, H. T. & M. C. Huang, 1992, A review of development of transgenic animal, Scientific Agriculture. 40:231-234. (in Chinese)
  • Wu, H. T., & M. C. Huang, 1991, Retroviral rectors for gene transfer in chicken. Scientific Agriculture. 39:128-135. (in Chinese)
  • Wu, H. T. & M. C. Huang, 1991, Theory and effects of electroporation for gene transfer. Scientific Agriculture. 39:256-264. (in Chinese)