National Chiayi University Web portal Department of Applied Chemistry Shu-Shi Chen

Shushi Chen

Title： Professor

Education： Ph.D., The University of Missouri- Rolla

Research Area： Analytic Chemistry, Chiral Stationary Phase Separation

Tel: 886-5-271-7997

Research Interests：

All of our research involves the development of new chiral stationary phase through modifying the commercially available chiral selector that are cheap such as antibiotics thiostrepton, rifamycin SV and the modification of mobile phase composition for enhancing the HPLC resolution of enantiomers. The results have been applied to the analysis of optical impurity of amino acids in the fermented food, artificial sweeteners and biological fluids such as urine and blood as functions of temperature, pH and concentration of enzyme, and to the enantiomeric enrichment of optically active pharmaceutical medicine such as b -blockers via solid-state extraction in large scale.

Our experimental and theoretical modeling and calculating efforts are aimed at better understanding the recognition site for LC chiral selector. Computer-aided modeling is used to re-generate the 3-D structure of immobilized molecule in search of its active site responsible for the chiral resolution based on the interaction calculations. We also apply this technique to aid the design of new chiral selectors or modify existing but cheap chiral molecules to be multimodal after their immobilization to the surface of silica gel for resolving different classes of optically active compounds via LC approach. These specially designed and modified chiral selectors, on the other hand, can be used as the solid phase in the solid-state extraction for large-scale enantiomeric enrichment or as the coating material for capillary tube used in electrophoresis and GC.

Publications：

1	Wang, Chao-Chiun; Chen, S; Wu, Tian-Shung 2003. “The Facile Reversed-Phase HPLC Resolution of Tetrahydrofurofuran Nucleus Lignans in Traditional Chinese Medicine: Quantitative Analysis of Asarinin and Sesamin in Asari Radix” –J. Chin. Chem. Soc., 50, 261-266.
2	Jou, Jen1-Huei; Chen, S; Wu, Tian-Shung 2003. “The Facile Reversed-Phase HPLC Resolution and Concentration Determination of Aristolachic Acid and Aristolactam Analogues in Traditional Chinese Medicine” –J. Liq. Chromatogr., 26, 3055-3065.
3	Chen, S. 2003. “The HPLC Enantiomeric Resolution of Phenyl Isothiocyanated Amino Acids on Teicoplanin Bonded Phase Using the Acetonitrile-Based Mobile Phase: A Structure Consideration”- J. Liq. Chromatogr., 26, 3467-3486. (NSC 91-2113-M-415-005-)
4	Chen, S. 2003. “The HPLC Enantioresolution of Phenyl Isothiocyanated Amino Acids on Vancomycin Bonded Phase Using the Acetonitrile-Based Mobile Phase: A Comparison to the Teicoplanin Phase”- J. Chin. Chem. Soc. 50, 1177-1182. (NSC 91-2113-M-415-005-)
5	Chen, S. 2003. “The HPLC Stereoselective Resolution of N-2,4-Dinitrophenylated (DNP) Amine-Containing Enantiomers on Teicoplanin Bonded Phase Using the Methanol-Based Mobile Phase”- Chromatographia 59, 173-180. (NSC 91-2113-M-415-005-)
6	Chen, S. 2004. “The Facile HPLC Enantioresolultion of Amino Acids on Naphthylethylcarbamate- b -Cyclodextrin Bonded Phases Using the Acetonitrile-Based Mobile Phase after Their Pre-column Derivatization with Phenyl Isothiocyanate : Factors That Affect the Resolution”- Amino Acids, 26, 291-298. (NSC 91-2113-M-415-005-)
7	Chen, S. 2004. “The Enantioresolution of Amino Acids, Amino Alcohols after Precolumn Derivatization with Phenyl Isocyanate on Naphthylethylcarbamate- b -Cyclodextrin Bonded Phases Using the Acetonitrile-Based Mobile Phase”- Chromatographia, 59, 696-703. (NSC 91-2113-M-415-005-)
8	Chen, S.; Ward, T. 2004. “Comparison of the Chiral Separation of Amino-Acid Derivatives by a Teicoplanin and RN- b -CD CSPs Using Waterless Mobile Phases: Factors That Enhance the Resolution”– Chirality, 16, 318-330. (NSC 91-2113-M-415-005-)
9	Chen, S. 2004. “The HPLC Enantioresolution of N-2,4-Dinitrophenylated (DNP) Amino Acids on Naphthylethylcarbamate- b -Cyclodextrin Bonded Phases Using the Acetonitrile-Based Mobile Phase ： Evidence for the Chiral Recognition Pattern”- Amino Acids , 27, 277-284. (NSC 91-2113-M-415-005-)
10	Chen, S. 2005.“The Factors That Influence the Elution Order for the Resolution of Amino Acids on Vancomycin Phase Using the Polar-Organic Mobile Phases after Their Precolumn Derivatization with Electrophilic Reagents”- Biomedical Chromatography, 19, 426-433 ?
11	Chen, S. 2005. “ The Enantioseparatin of Amino Acids on a Teicoplanin Chiral Stationary Phase Using Non-Aqueous Mobile Phases after Pre-Column Derivatization with Sulfur-Containing Reagents: the Considerations of Mobile Phase Composition and Analyte Structure Variation on Resolution Enhancement ”- Biomedical Chromatography (accepted)
12	Chen, S. 2005. “ The Influence of Steric Hindrance from the Alkyl Group of Isothiocyanate Reagent on the Enantioresolution of the Amino Acids after Their Derivatization Using a Teicoplanin CSP under the Elution of the Methanol-Based Solvent Mixture ”- Chromatographia (accepted)
13	Chen, S. 2005. “ The Enantioseparatin of Amino Acids on a Teicoplanin Chiral Stationary Phase Using Non-Aqueous Mobile Phases after Pre-Column Derivatization with Sulfur-Containing Reagents: the Considerations of Mobile Phase Composition and Analyte Structure Variation on Resolution Enhancement ”- Biomed. Chromatogr , 20, 718-728
14	Hsieh, T.J. and Chen, Shushi. 2006. “ A Fluorescent Electrophilic Reagent, 9-Fluorenone-4-carbonyl Chloride (FCC), for the Enantioresolution of Amino Acids on a Teicoplanin Phase under the Elution of the Methanol-Based Solvent Mixture ”- Amino Acids, 33, 97-104
15	Hsieh, T.J. and Chen, Shushi. 2006 “ A facile HPLC method for optical purity and quantitative measurements of phenylalanine from the hydrolyzed aspartame under different pH and temperature after its derivatization with a fluorescent reagent ”- Amino Acids, 33,123-128
16	Hsiao, Ya-Li and Chen*, S. 2009 “ LC Separation of Enantiomers on Silica-Bonded Thiostrepton Derivatives ”- Chromatographia, 70,1031-1038
17	Yeh, Jian-De and Chen*, S. 2011 “Isocratic Separation of Dansylated Biogenic Amines on a C(8)-Bonded Silica Column under the LC Elution of Methanol-Based Mobile Phase”- J. Chin. Chem. Soc. , 58,241-246
18	Yeh, Jian-De and Chen*, S. 2012 “ Heavy Metallic and Organometallic Ions Scavenging Using Silica-Based Adsorbent Functionalized with Ligands Containing Sulfur and Nitrogen Elements ”- J. Chin. Chem. Soc. , 59,98-106
19	Cheng-Sheng Chen and Shushi Chen* 2013 “Adsorption of Pesticidal Compounds Bearing a Single Carboxyl Functional Group and Biogenic Amines by Humic Fraction-Immobilized Silica Gel”- Journal of Agricultural and Food Chemistry,11,3600-3610
20	Yan-Shuo Lai, Cheng-Shung Chen, and Shushi Chen* 2013 “ Adsorption and Desorption of Plasticizers with Humic-Fraction-Immobilized Silica Gel in Hexane: A Facile Approach to Pre-concentration”- Journal of Agricultural and Food Chemistry, 5,5286-5290
21	Yan-Shuo Lai and Shushi Chen* 2013 “ The Adsorption of Organophosphate Pesticides with Humic Fraction-Immobilized Silica Gel in Hexane ”- Journal of Chemical and Engineering Data, 12, 2290-2301
22	Shun-Wei Hu and Shushi Chen* 2013 “ Adsorption of Triazine Derivatives with Humic Fraction-Immobilized Silica Gel in Hexane: A Mechanistic Consideration ”- Journal of Agricultural and Food Chemistry, 9, 8524-8532
23	Shun-Wei Hu andShushi Chen* 2014 “ Adsorption/Desorption of Pesticidal Carboxylate Esters by Switching Liquid Phases on Humic-Fraction-Modified Silica Gel Matrix ”- JOURNAL OF THE CHINESE CHEMICAL SOCIETY, 61, 1015-1024
24	Shun-Wei Hu andShushi Chen* 2015 “ A Multipurpose Lignin-based Adsorbent for Metallic Ions, Nanoparticles and Various Organophosphate Pesticides in Hexane ”- JOURNAL OF THE CHINESE CHEMICAL SOCIETY, 62, 875-888
25	陳世晞 2015 “ 萃取技術的回顧 ”- 化學季刊, 73, 79-92
26	陳世晞 2016 “ 以部份腐植酸修飾之矽膠吸附三氮六環類似物並結合支撐式液膜萃取裝置之製備型應用 ”- 化學季刊, 74, 79-96